monarch dna gel extraction kit (New England Biolabs)
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monarch dna gel extraction kit
Monarch Dna Gel Extraction Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1469 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/monarch+dna+gel+extraction+kit/Monarch+Spin+DNA+Gel+Extraction+Kit/pmc13657075-62-9-14
Average 99 stars, based on 1469 article reviews
Monarch Dna Gel Extraction Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1469 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/monarch+dna+gel+extraction+kit/Monarch+Spin+DNA+Gel+Extraction+Kit/pmc13657075-62-9-14
Average 99 stars, based on 1469 article reviews
monarch dna gel extraction kit - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis"
Article Title: Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis
Journal: MicrobiologyOpen
doi: 10.1002/mbo3.70435
Figure Legend Snippet: Optimization of RPA primers and CRISPR/Cas12a detection for the Mycoplasma bovis uvrC gene. (A) Schematic of p‐uvrC generation by restriction cloning of a 558‐bp uvrC fragment, which was used as the standard DNA template. (B) Screening of eight RPA primer pairs (PP1–PP8) targeting uvrC; PP3–PP8 produced strong, specific amplicons, and target amplification was also detected in DNA from M. bovis ‐positive bison lung. (C) Sequence of three CRISPR guide RNAs (gRNA1–gRNA3) designed within the amplified uvrC region adjacent to 5′‐TTTG‐3′ PAM sites. (D) Comparison of Cas12a fluorescence kinetics showed that all three guides detected uvrC, with gRNA3 producing the fastest and strongest signal. (E) Optimization of ssDNA‐FQ reporter concentration identified 0.75 μM as the best condition for visual detection with minimal background, although maximal fluorescence was reached at 5 μM. (F) All guide RNA combinations produced detectable fluorescence with similar kinetics, although gRNA2 + 3 and gRNA1 + 2 + 3 showed slightly faster onset.
Techniques Used: CRISPR, Cloning, Screening, Produced, Amplification, Sequencing, Comparison, Fluorescence, Concentration Assay
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